A new FACS approach isolates hESC derived endoderm using transcription factors.

A new FACS approach isolates hESC derived endoderm using transcription factors.

Blog Article

We show that high quality microarray gene expression profiles can be obtained following FACS sorting of apac1/60/1/cw cells using combinations of transcription factors.We use this transcription factor FACS (tfFACS) methodology to perform a genomic analysis of hESC-derived endodermal lineages marked by combinations of SOX17, GATA4, and CXCR4, and find that triple positive cells have a much stronger definitive endoderm signature than other combinations of these markers.Additionally, SOX17(+) GATA4(+) cells can be obtained at a much earlier stage of differentiation, kicker pro comp 10 prior to expression of CXCR4(+) cells, providing an important new tool to isolate this earlier definitive endoderm subtype.Overall, tfFACS represents an advancement in FACS technology which broadly crosses multiple disciplines, most notably in regenerative medicine to redefine cellular populations.